bleomycin sulfate  (MedChemExpress)

Journal: In Vivo

Article Title: Antagonistic Effect of Erythropoietin Receptor Signaling in Macrophages and Type II Alveolar Epithelial Cells on Pulmonary Fibrosis in Mice

doi: 10.21873/invivo.14302

Figure Lengend Snippet: Knockout of macrophage EPOR ameliorates pulmonary fibrosis in mice. (A) H&E staining was used to assess the degree of pulmonary pathological changes bar=625μm, n=4. (B) Masson staining was used to evaluate the degree of collagen deposition in the lungs. bar=625μm, n=4. (C) Quantification of lung hydroxyproline levels.n=4. (D) The protein level of EPOR in the lung was measured by western blot. n=3. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. MΦ-EPORcko: Bleomycin-treated macrophage-specific EPOR knockout mice; EPOR-C: bleomycin-treated EPOR-KOloxp homozygous mice; α-SMA: α-smooth muscle actin; Col-1: collagen I.

Article Snippet: The reagents used are as follows: Bleomycin (MCE, USA, #HY-17565), Fetal Bovine Serum (Hyclone, Logan, UT, USA, #SH30070), Dulbecco’s Modified Eagle Medium (Hyclone, #SH30022), Cell Dissociation Solution (Gibco, Waltham, MA, USA, #13151014), Hematoxylin-Eosin (H&E) Stain Kit (Solarbio, Beijing, PR China, #G1120), Masson’s Trichrome Stain Kit (Solarbio, #G1346), RNA Fast 200 Kit (Fastagen, Shanghai, PR China, #220011), RT Reagent Kit with gDNA Eraser (Takara, Shiga, Japan, #RR047A), SYBR Green qPCR Mix (MCE, USA, #HY-K0501), Citrate Antigen Retrieval Solution (Beyotime, Shanghai, PR China, #P0083), Bradford Protein Quantification Kit (Beyotime, #P0006C), DAPI (Biosharp, Beijing, PR China, #BL105A), TGF-β1 (Sino Biological, Beijing, PR China, #10804-HNAC).

Techniques: Knock-Out, Staining, Western Blot

Journal: In Vivo

Article Title: Antagonistic Effect of Erythropoietin Receptor Signaling in Macrophages and Type II Alveolar Epithelial Cells on Pulmonary Fibrosis in Mice

doi: 10.21873/invivo.14302

Figure Lengend Snippet: Knockout of type II alveolar epithelial cells EPOR exacerbates pulmonary fibrosis in mice. (A) H&E staining was used to assess the degree of pulmonary pathological changes. Bar=625 μm, n=4. (B) Masson staining was used to evaluate the degree of collagen deposition in the lungs. Bar=625 μm, n=4. (C) Quantification of lung hydroxyproline levels, n=4. (D) The protein level of EPOR in the lung was measured by western blot, n=3. *p<0.05, **p<0.01, ****p<0.0001. Sftpc-EPORcko: Bleomycin-treated type II alveolar epithelial cells-specific EPOR knockout mice; EPOR-C: bleomycin-treated EPOR-KOloxp homozygous mice; α-SMA: α-smooth muscle actin; Col-1: collagen I.

Article Snippet: The reagents used are as follows: Bleomycin (MCE, USA, #HY-17565), Fetal Bovine Serum (Hyclone, Logan, UT, USA, #SH30070), Dulbecco’s Modified Eagle Medium (Hyclone, #SH30022), Cell Dissociation Solution (Gibco, Waltham, MA, USA, #13151014), Hematoxylin-Eosin (H&E) Stain Kit (Solarbio, Beijing, PR China, #G1120), Masson’s Trichrome Stain Kit (Solarbio, #G1346), RNA Fast 200 Kit (Fastagen, Shanghai, PR China, #220011), RT Reagent Kit with gDNA Eraser (Takara, Shiga, Japan, #RR047A), SYBR Green qPCR Mix (MCE, USA, #HY-K0501), Citrate Antigen Retrieval Solution (Beyotime, Shanghai, PR China, #P0083), Bradford Protein Quantification Kit (Beyotime, #P0006C), DAPI (Biosharp, Beijing, PR China, #BL105A), TGF-β1 (Sino Biological, Beijing, PR China, #10804-HNAC).

Techniques: Knock-Out, Staining, Western Blot